Biosynthesis of P-GlycoproteinEdit
P-glycoprotein is commonly been shown to be a constitutively active gene. However, upregulation of the gene can occur through a complex array of environmental signals and stresses. Most regulation that occurs on the P-gp gene has been shown to be mostly positive, as the gene lacks a specific repressor.
The transcription of P-gp is enhanced by an upstream sp1 binding site. Sp1 is present near many genes in which the promoter lacks a TATA box. Deletions of the sp1 binding region have shown a 6 fold decrease in transcription.
Cancer has also been shown to upregulate P-gp, causing cells to efflux at a much higher rate than normal.
Synthesis in HumansEdit
The specific gene sequence of P-Glycoprotein in humans can be found HERE
P-gp is synthesized in most Human cells, however there tends to be a higher accumulation in epithelial cells of the small and large intestines, brain, kidneys and liver. It is predicted that these occur due to the stress of these systems.
- Yuzo: P-Glycoprotein: Introduction
- Yuzo: P-Glycoprotein: Biological function
- Yuzo: P-Glycoprotein: Biosynthesis
- Yuzo: P-Glycoprotein: Gene sequence
- Yuzo: P-Glycoprotein: Amino acid sequence and composition
- Yuzo: P-Glycoprotein: Domains and structural motifs
- Yuzo: P-Glycoprotein: Interactions with macromolecules and small molecules
- Yuzo: P-Glycoprotein: Molecular biodiversity and evolution
- Yuzo: P-Glycoprotein: Literature overview
- Yuzo: P-Glycoprotein: Online resources
- ↑ Scotto, K.W., and Egan, D.A. (1998). Transcriptional regulation of MDR genes. Cytotechnology 27, 257–269.
- ↑ M M Cornwell, D.E.S. (1993). SP1 activates the MDR1 promoter through one of two distinct G-rich regions that modulate promoter activity. The Journal of Biological Chemistry 268, 19505–19511.